《植物生理学报》 2009, 45(12): 1181-1185

亚麻胚性细胞悬浮培养体系的建立和影响因素

苗书魁¹, 姜丽², 计巧灵^1,*, 李文婷¹, 热西旦· 尼格买提¹

1 新疆大学生命科学与技术学院, 乌鲁木齐830046; 2 新疆轻工职业技术学院食品工程系, 乌鲁木齐830021

通信作者：计巧灵；E-mail: wji1118@yahoo.com.cn

摘　要：

亚麻品种‘双亚五号’的胚性愈伤组织诱导最佳培养基为MB (MS无机盐加B5维生素)+1.0 mg·L-1 2,4-D; 细胞初始悬浮培养的最佳培养基为MB+0.2 mg·L-1 6-BA; 细胞继代悬浮培养的最佳培养基为改良的MB (NH4NO3 减半, KNO3 加倍)+0.02 mg·L-1 2,4-D+0.2 mg·L-1 6-BA; 适宜的蔗糖量为50 g·L-1; 适宜的继代接种量为1.0~1.5 g·(25 mL)-1; 继代间隔为5~7 d。

关键词：亚麻; 胚性愈伤组织; 悬浮细胞系

收稿：2009-09-23   修定：2009-12-01

资助：国家自然科学基金(30660095)和新疆教育厅重点科研项目(XJEDU2006108)。

Establishment and Influence Factors of Embryogenic Cell Suspension Lines of Linum usitatissimum L. in vitro

MIAO Shu-Kui¹, JIANG Li², JI Qiao-Ling^1,*, LI Wen-Ting¹, Rexidan·Nigemaiti¹

¹College of Life Science and Technology, Xinjiang University, Urumqi 830046, China; ²Food Engineering Department, Xinjiang Institute of Light Industry Technology, Urumqi 830021, China

Corresponding author: JI Qiao-Ling; E-mail: wji1118@yahoo.com.cn

Abstract:

The optimum induction medium for the embryogenic callus of Linum usitatissimum ‘Shuangya No. 5’ was MB (MS inorganic salts supplemented with B5 vitamins)+1.0 mg·L^-1 2,4-D. The optimum medium for initial culture of suspension cells was liquid MB supplemented with 0.2 mg·L^-1 6-BA. The suspension cells were cultured on modified MB liquid medium NH₄NO₃ halved, KNO₃ doubled) supplemented with 0.02 mg·L^-1 2,4-D+0.2 mg·L^-1 6-BA for subculturing. The suitable sucrose concentration was 50 g·L^-1. The optimal inoculative densities were 1.0–1.5 g·(25 mL)^-1, and the subculture period was 5–7 d.

Key words: Linum usitatissimum; embryogenic callus; cell suspension lines